Response of the structure of rat kidney and inflammatory factors to Blackcurrant Extract at 24 hours after exhaustive exercise

doi:10.3969/j.issn.2095-4344.2014.49.026

Abstract

Abstract:

BACKGROUND: The kidney tissues easily affected exercise ischemia reperfusion, increased free radicals and inflammation, resulted in abnormal renal function after acute exercise.

OBJECTIVE: To observe the influence of Blackcurrant Extract on the structure of kidney and expression of tumor necrosis factor-α and nuclear factor-κB at 24 hours after exhaustive exercise.

METHODS: A total of 30 male Wistar rats were randomly divided into three groups (n=10). Rats in the Blackcurrant Extract group were intragastrically administered 0.44 g/kg Blackcurrant Extract. Rats in the quietness control group and 24-hour exhaustive exercise group were intragastrically given an equal volume of distilled water for 6 consecutive weeks. Rats in the 24-hour exhaustive exercise group and Blackcurrant Extract group received no swimming motion until exhaustion fatigue after final intragastric administration. Twenty-four hours later, samples were obtained. Kidney tissue morphology and ultrastructure were observed by electron microscopy and light microscopy. Protein expression of tumor necrosis factor-α and nuclear factor-κB was detected using immunohistochemistry. Tumor necrosis factor-α mRNA and nuclear factor-κB mRNA expression was detected using RT-PCR.

RESULTS AND CONCLUSION: Compared with the quietness control group, tumor necrosis factor-α protein and nuclear factor-κB protein expression in the kidney was higher in the 24-hour exhaustive exercise group, and tumor necrosis factor-α mRNA and nuclear factor-κB mRNA expression was significantly increased (P < 0.01). Compared with the 24-hour exhaustive exercise group, tumor necrosis factor-α protein and nuclear factor-κB protein expression was lower in the Blackcurrant Extract group, and nuclear factor-κB mRNA expression was significantly decreased (P < 0.05); tumor necrosis factor-α mRNA expression was significantly reduced (P < 0.01). Kidney in the 24-hour exhaustive exercise group showed obvious morphological changes and ultrastructural damage. The structure of the kidney in the Blackcurrant Extract group tended to be normal. Results suggested that Blackcurrant Extract can repair the kidney tissue injury, reduce the expression of inflammatory factors, and prevent inflammatory damage in the kidney at 24 hours after exhaustive exercise.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: ribes, exercise, kidney, NF-kappa B, tumor necrosis factor-alpha

CLC Number:

R318

Liu Xia, Jiang Jia-ye, Lu Hai-ying, Gu Cui-ying, Cai Mei-qin. Response of the structure of rat kidney and inflammatory factors to Blackcurrant Extract at 24 hours after exhaustive exercise [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(49): 8020-8025.

Figures/Tables 2

2.1 实验动物数量分析参加实验大鼠有30只，中途有3只大鼠游泳死亡，其中力竭运动后24 h组2只，黑加仑提取物组1只，进入结果分析共计27只。 2.2 黑加仑提取物对肾脏结构的影响 2.2.1 光镜观察结果安静对照组肾脏组织着色均匀，结构清晰致密，核大小均匀(图1A)。力竭运动后24 h组肾小管上皮细胞出现疏松化，重度空泡变性，肾间质少量炎细胞浸润(图1B)。黑加仑提取物干预后肾组织少数肾小管上皮细胞空泡变性，较力竭运动后24 h组有明显改善(图1C)。 2.2.2 电镜观察结果安静对照组肾小管微绒毛排列整齐(图2A)。力竭运动后24 h组中系膜区增宽，肾小管微绒毛排列紊乱，肾小管上皮细胞空泡变性，部分肿胀，溶酶体吞噬(图2B)。黑加仑提取物组较力竭运动后24 h组有改善，肾小管微绒毛排列稍紊乱，系膜区基质增生(图2C)。 2.3 黑加仑提取物对肾脏组织中核因子κB蛋白和肿瘤坏死因子α蛋白表达的影响安静对照组大鼠肾脏细胞胞浆未见任何染色的阳性颗粒，核因子κB蛋白和肿瘤坏死因子α蛋白呈阴性表达(图3A，4A)；力竭运动后24 h组大鼠肾脏细胞胞浆内可见棕黄色阳性颗粒数明显增多，着色较深，核因子κB蛋白和肿瘤坏死因子α蛋白呈阳性表达(图3B，4B)，尤以病灶及其周边部位明显；黑加仑提取物组肾脏细胞胞浆内阳性颗粒数明显减少，着色变浅(图3C，4C)。 2.4 补充黑加仑提取物对肾脏组织中核因子κB mRNA和肿瘤坏死因子α mRNA表达的影响与安静对照组比较，力竭运动后24 h组核因子κB mRNA和肿瘤坏死因子α mRNA表达水平极显著增加(P < 0.01)；与力竭运动后24 h组比较，黑加仑提取物组核因子κB mRNA表达水平显著性降低(P < 0.05)；肿瘤坏死因子α mRNA表达水平极显著性下降(P < 0.01)。黑加仑提取物组核因子κB mRNA和肿瘤坏死因子α mRNA表达水平与安静组相比差异均无显著性意义(P > 0.05)，见表2。"

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